chromotography : LUSENET : PID Controller Tuning : One Thread

i would like to know about the theory and method of chromotography.kindly answer me

-- S.Hemantha Kumar (, January 04, 2004


please could you tell me a brief bit of infomation on what chromotography is

-- andy verrier (, January 28, 2004.

you dumbass chromatography is a method of sepaarating subtances that normally would be difficult to seperate

-- frank smith (, February 04, 2004.

Chromotography is a method of separating substances based on their molecular sizes, for example in a paper chromotography, one would place the substance near the botom of the paper. Then emmerse the botom in a solvent (varies depending on what you are chromotizing). Depending on the substance, it will take about 30 minuts to a few days. The substances separate out and you have a piece of the paper wiht each individual component on it. (if this went over your head please email me and i will try to explain it better)

-- shawn (, February 17, 2004.

but i understand thear are difrent typs of crhromotography like gas liquid and bye the way i new what u said u didnt give me that much help but if u answer my ? then that proves that ur realy smart ha

-- Alexia (, February 22, 2004.

Chromatography is a separations method that relies on differences in partitioning behavior between a flowing mobile phase and a stationary phase to separate the the components in a mixture.

A column (or other support for TLC, see below) holds the stationary phase and the mobile phase carries the sample through it. Sample components that partition strongly into the stationary phase spend a greater amount of time in the column and are separated from components that stay predominantly in the mobile phase and pass through the column faster.

As the components elute from the column they can be quantified by a detector and/or collected for further analysis. An analytical instrument can be combined with a separation method for on-line analysis. Examples of such "hyphenated techniques" include gas and liquid chromatography with mass spectrometry (GC-MS and LC-MS), Fourier-transform infrared spectroscopy (GC-FTIR), and diode-array UV-VIS absorption spectroscopy (HPLC-UV-VIS).

-- Wendy (, April 01, 2004.

frank smith yr the dumb ass u piece of crap S.Hemantha Kumar was jus askin a question u knw my email. email me bck if u got something to say fool

-- Ash712 (, July 08, 2004.

i know hardly anything about this crap and i had to do a 5 page report thanks alot for nothing! why don't u try putting in teen lingo!!

-- ChuKiE (, October 12, 2004.

Tell me more about how a chromatography works. 1) Especially how it separates Amino acids? 2) The difference between tin layer and paper chromatography and the advantage of each. 3) Best and simplest way of running the chromatogram?

Thank you very much for your cooperation!

-- Seble Haile (, October 18, 2004.

what? i dont get it???

-- limola levern (, March 01, 2005.

omg i'm still in skool and i know that but i'm not gonna tell u coz see its this thing called a dictionary its a book rite? its normally thick coz its full of these things called definitions which are descriptions of wat words mean so GET ONE AND LOOK IN THE D SECTION

-- wouldn'tulike2know (, March 03, 2005.

Chromotography is a method used by chemists to seperate and identify the components of a mixture. There are numerous different types of chromotography all depending on the equilibrium set up when a compound distributes itself between two phases, the static or stationary phase and the mobile phase. different compounds move along with the mobile phase at different speeds. Thin layer chromotography - a small sample of a mixture is spotted onto a solid support material such as silica gel spread in a uniform layer on a glass or plastic plate (the static phase) and a suitable solvent rises up the plate (mobile phase)carrying the compounds of the mixture with it. When you examine the developed plate a series of spots will determine the compounds of the mixture.

-- Alex Ward (, March 05, 2005.

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